About the Practical Course
The field of genome assembly has been revolutionized by the availability of long reads (Nanopore, PacBio) and scaffolding approaches using chromosome conformation capture (HiC), making it possible to generate chromosome-scale assemblies for virtually any organism. This practical course will focus on these developments, with the aim of equipping each participant with the theoretical knowledge and practical know-how to be able to sequence their genome of interest and analyze it, all the way to publication. Reference genomes are becoming a new fundamental tool in biodiversity and evolutionary research and accordingly, the number of reference genome initiatives is rising, as exemplified by the growing number of international initiatives aimed at increasing the availability of genomes across the tree of life such as the Earth BioGenome Project.
The course includes practical training in the use of computational tools for genome assemblies such as HiFiasm, Flye, as well as NextDeNovo and for genome annotation such as GeMoMa, Braker, and Maker. The strengths and weaknesses of each of them will be discussed after its use. More importantly, the students will be trained in the use and interpretation of tools to perform Quality Control (QC) at each step, allowing them to discuss the quality of the results and possible strategies to resolve assembly problems and increase the quality of the assemblies.
The course is aimed at graduate students and postdoctoral researchers, but applications from scientists at any stage of their careers are encouraged.
About EMBO Courses and Workshops
EMBO Courses and Workshops are selected for their excellent scientific quality and timelines, provision of good networking activities for all participants and speaker gender diversity (at least 40% of speakers must be from the underrepresented gender).
Organisers are encouraged to implement measures to make the meeting environmentally more sustainable.