About the Practical Course
Cryo electron microscopy (cryo EM) is a major structural biology method for studying macromolecular complexes and cellular structures in their native states. Stable high resolution cryo microscopes, direct electron detectors and automated systems for data collection now allow routine biomolecular structure determination at near-atomic resolution using both single-particle and tomography approaches. Atomic models are routinely built or fitted into cryo EM maps revealing interactions of their components and conformations of different functional states. On the other hand, hybrid approaches combining EM with optical microscopy and Scanning EM allow unprecedented insight into targeted cellular structures. Cryo-EM is therefore very powerful as it provides structural information across a wide range of sizes and resolution, bridging from molecules to cells. It can moreover be used to sort out heterogeneous conformations to capture steps in the operation of macromolecular machines.
The aim of this EMBO Practical Course is to teach the basic principles and practical aspects of image processing to structural and molecular biologists wishing to determine macromolecular structures by cryo EM. The course will concentrate on processing of high-resolution image data, for both single particle images and tomograms, and will be aimed at advanced PhD students and postdocs who use cryo EM for structural analysis.
Image credits: Kévin Macé, Gabriel Waksman, Sander Van der Verren and Giulia Zanetti
About EMBO Courses and Workshops
EMBO Courses and Workshops are selected for their excellent scientific quality and timelines, provision of good networking activities for all participants and speaker gender diversity (at least 40% of speakers must be from the underrepresented gender).
Organisers are encouraged to implement measures to make the meeting environmentally more sustainable.