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EMBO Practical Course

3D developmental imaging

01 – 09 July 2022 | Oeiras, Portugal

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  • Registration Deadline (in-person participation, including practicals)
  • 19 May 2022
  • Chosen In-Person Participants Will Be Notified By
  • 25 May 2022
  • Registration Deadline (virtual participation, no practicals)
  • 17 June 2022
  • Payment Deadlines
  • 3 June 2022 (in-person)
    24 June 2022 (virtual)

Apply now!

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About the Practical Course

Practical Course illustration

3D microscopy and mesoscopy are of major interest for developmental biologists, and have seen tremendous technological developments in the last years. Obtaining high quality, artefact-free multidimensional images and analysing them is now easier but still a challenge and the techniques are still not addressed in academic curricula or the expertise is still lacking in smaller labs/facilities. This EMBO practical course is aimed at junior researchers in Developmental Biology, struggling with 3D imaging of cell movement and tissue morphogenesis in embryos. Confocal, two-photon, light-sheet/SPIM and optical tomography (mesoscopy) are featured in the course. Open-source initiatives and commercial partners contribute with state-of-the-art solutions, which are available to the participants during the course to test with their own samples. Visualisation, processing, analysis and publication of multidimensional images is also addressed, with both commercial and open source tools. The 9 day programme includes morning lectures with several renowned experts, and 6-8h of practicals/day with the support of ~20 tutors and more than 10 high-end 3D imaging techniques. It also includes "micro"workshops on DIY light-sheet and optical tomography, and 3D visualisation and image analysis workflow design.

This year we will make the event “hybrid”, with i) lectures in “webinar” format broadcast to everyone who qualifies and registers before June 15th (“virtual” attendance) and ii) practical sessions only for the selected students who propose projects (= “in person”). ATTENTION: to be considered for selection for “in person” attendance, applicants must submit the application and project BEFORE April 15th and be ready to bring samples to the course (student participation is mandatory for all days, no exceptions!). During the registration form, please make the appropriate selection when asked “I will attend the meeting:”. There will be 16 seats available for “In person” attendance, and 100 for “virtual attendance”. We hope that with this format we can reach a broader audience.

Different registration charges apply to both participations.
i) In person attendance is 200€ registration (includes all sessions, theoretical and practical, all course materials, food & lodge, local transportation and access to all equipment featured in the course). A number of travel grants are available (more info here).
ii) “Virtual-only” access costs 20€ and gives access to all webinars, participation on one “I need help!” session with our tutors (requests must be submitted in advance and will be pre-selected), participation on a “virtual get together”, remote access to equipment demos (upon request only, and limited spots available), and a participation certificate (after completion of a final questionnaire).

Images were obtained during the previous edition of the course







  • Registration Deadline (in-person participation, including practicals)
  • 19 May 2022
  • Chosen In-Person Participants Will Be Notified By
  • 25 May 2022
  • Registration Deadline (virtual participation, no practicals)
  • 17 June 2022
  • Payment Deadlines
  • 3 June 2022 (in-person)
    24 June 2022 (virtual)



The registration for in-person participation covers the costs for:

  • Accommodation and meals during the course
  • Local transportation
  • Social event + gala dinner
  • Course manual and lab supplies
  • Access to >10 instruments, workstations/software (w/ scheduling)
  • 2Tb portable hard drive + temp software licenses (from sponsors)


Participants will be sent payment details by email, upon selection.

Selection criteria

  • Motivation and adequacy of project proposed (must be related to morphogenesis and require 3D imaging)
  • Geographic distribution
  • Model (organism) proposed

Any other registration information

Motivation letter is required, and explanation of the project, organism model and type of imaging that the applicant proposes to bring to the course.

Travel Grants and Registration Fee Waivers

A limited number of travel grants and registration fee waivers are available for participants.

Applicants do not need to apply separately for these but should indicate on the registration form if they wish to be considered.

Selection of awardees is handled directly by the organizers. Priority is given to participants of any nationality working in laboratories in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Malta, Lithuania, Poland, Portugal, Slovakia, Slovenia, Spain and Turkey, as well as from EMBC Associate Member States Chile, India, Singapore and Taiwan. More information is available on the EMBO Travel Grants' page.

Child care grant

EMBO Courses and Workshops offers grants to offset additional child care costs incurred by participants or speakers when participating at any EMBO Courses and Workshop funded meeting. Eligible costs include fees for a caregiver or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please indicate on the registration form whether you would like to be considered for the grant. Please also describe how you intend to use the child care grant and specify the sum that you will need.






Instituto Gulbenkian de Ciencia

Rua da Quinta Grande N6
2780-156 Oeiras, Portugal


All participants will be lodged at a local hotel. Students will share double beds rooms. Transportation to and from the hotel to the venue is included.


Instructions to get to Oeiras and the IGC:

Oeiras is halfway in between the Lisbon-Cascais railway. There is a shuttle bus service from the Lisbon airport to “Cais-do-Sodre”. There is also a subway; you start on the red line and then switch to the green line at Alameda. From Cais-do-Sodre catch the train headed to Cascais (every 20/30min) and all trains will stop in Oeiras (~20min ride), where the IGC is located. If when arriving you go straight to the hotel, it’s only 1 more station - Carcavelos. From the hotel to the IGC catch the same train but headed towards Lisbon and exit in Oeiras (1 train stop).

From the Oeiras train station to the IGC

Exit the Oeiras station and head towards the taxi/bus park across the garden towards the pharmacy and restaurants (headed right); keep following that sidewalk for ~400m until you reach the traffic lights. Turn left, cross over the pedestrian crossing and you are at the IGC's main entrance.

Follow the link for a more detailed and interactive google map of the buildings and how to get to the IGC from the train station.

About the Area

Local transportation from and to hotel is provided daily for all students and trainers.

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