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EMBO Practical Course

Characterization of macromolecular complexes by integrative structural biology

28 May – 04 June 2022 | Grenoble, France

EMBO Childcare Grants Logo

  • Registration Deadline
  • 31 January 2022
  • Chosen Participants Will Be Notified By
  • 31 March 2022
  • Payment Deadline
  • 15 May 2022

Apply now!

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About the Practical Course

This EMBO Practical Course aims to teach participants how to integrate structural biology approaches to expedite characterization of large macromolecular complexes from the atomic to cellular scale. Lectures and practical sessions will explain the techniques used to produce, purify, reconstitute and characterize multi-subunit protein and protein/nucleic acid complexes for structural analysis.

Specifically, this EMBO Practical Course will cover multi-subunit protein expression in bacteria, insect and mammalian cells; production, folding, and purification of non-coding RNAs; affinity, electrophoretic and centrifugation methods to isolate and purify sub-complexes and holocomplexes; biochemical and biophysical experiments that inform on the stoichiometric and conformational homogeneity of complexes; and strategies for determining the overall 3D architecture of large macromolecular assemblies and ultimately their structure at atomic or pseudo-atomic resolution.

Special emphasis will be placed on:

(i) the latest advances in producing targets directly from endogenous or recombinant sources or from cell free approaches (including production of RNA by in vitro transcription), and in reconstituting complexes from individually purified components or sub-complexes;

(ii) techniques for assessing and improving sample quality (homogeneity, stoichiometry, monodispersity); and

(iii) integration of structure determination methods (serial crystallography, cryo-electron microscopy, small-angle scattering, NMR, atomic force microscopy, native mass spectrometry, FRET, super-resolution light microscopy) to obtain a multi-resolution view of a given complex.

 

Speakers

 

Programme

 

Registration

  • Registration Deadline
  • 31 January 2022
  • Chosen Participants Will Be Notified By
  • 31 March 2022
  • Payment Deadline
  • 15 May 2022

 

  • STUDENT/POSTDOCS EUR 350
  • ACADEMIC EUR 350
  • INDUSTRY EUR 1000

Registration includes:

  • Accommodation for 8 nights (May 28th to June 4th)
  • Food
  • Course material

Payment

Payment instructions will be sent to successful applicants once the selection procedure is complete.

Selection criteria

1. Priority to advanced PhD students and early-stage postdocs

2. Priority to applicants working on a challenging structural biology project involving a multi-subunit macromolecular complex


3. Preference for applicants (of any nationality) working in EMBC labs; a limited number of applicants based in non-EMBC Member States will also be accepted

Poster specifications

Posters should preferably be printed in size A0.

Travel grants

A limited number of travel grants are available for participants. Applicants do not need to apply separately for travel grants for this event but should indicate on the registration form if they wish to be considered for a travel grant. Selection of awardees is handled directly by the organizer who will notify all eligible participants. More information is available at EMBO Travel Grants' page.

Child care grant

EMBO Courses and Workshops offers grants to offset additional child care costs incurred by participants or speakers when participating at any EMBO Courses and Workshop funded meeting. Eligible costs include fees for a caregiver or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please indicate on the registration form whether you would like to be considered for the grant. Please also describe how you intend to use the child care grant and specify the sum that you will need.

 

Contact

Angelika Thomasson

Email: athomasson@embl.fr

Phone: 0033-(0)47620-7123

Marco Marcia

Email: mmarcia@embl.fr

Phone: 0033-(0)47620-7759

 

 

Venue

EPN Campus

71 avenue des Martyrs

38042 Grenoble

France

Accommodation

The exact lodging situation will be communicated as soon as possible.

Students will be lodged in single rooms and in agreement with all COVID-19 national regulations in place at the time of the course. Should the medical situation not allow for regular in person attendance an alternative programme for a virtual course will be prepared.

Transport

Details on reaching the EPN campus by train, plane or car can be found here.

About the Area

Touristic information regarding the city of Grenoble and its surroundings can be found here.

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