About the Practical Course
This EMBO Practical Course will cover the use of small angle scattering (SAS) of both neutrons and X-rays for the determination of the structures of biological macromolecules. Particular attention will be paid to sample preparation and the analysis and interpretation of SAS data in a biological context. The aim of this course is to enable the participants to maximise the information gained from the SAS technique in their future experiments.
Small angle scattering is a powerful technique for determining the structures of biological molecules in solution. Not only can small angle scattering provide precise information concerning the size, molecular weight, volume and shape of single molecules but it can also provide detailed information from large, multi-component macromolecular complexes. For these purposes the scattering properties of neutrons are particularly well suited. Through the use of specific isotope labeling techniques and solvent contrast variation it is possible to determine the structural organisation of specific components within a large macromolecular assembly.