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EMBO Practical Course

CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and cellular tomography

19 – 30 January 2020 | Kolkata, India

EMBO Childcare Grants Logo
  • Registration Deadline
  • 15 September 2019
  • Abstract Submission Deadline
  • 30 September 2019
  • Chosen Participants Will Be Notified By
  • 15 October 2019
  • Payment Deadline
  • 30 October 2019

Apply now!

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About the Practical Course

Practical Course illustration

Structural Biology using Single Particle Cryo Electron Microscopy (SP CryoEM) has become a major tool for studying macromolecular assemblies. Visualising unprecedented details of cellular components, and solving in-situ protein structures are now possible with advancement in CryoEM methods. Developments in Field Emission Gun (FEG) Transmission Electron Microscope (TEM) and other TEM hardware, CryoEM specimen preparation methods, automated data acquisition systems, Direct Electron Detectors and improved image processing softwares have led to ~ 2000 proteins/protein-DNA/RNA complex structures larger than 52 kDa in size being routinely solved with < 4 Å resolution. The highest resolution obtained so far for a single particle EM map deposited in Electron Microscopy Data Bank (EMBD) is 1.54 Å. However these cutting edge CryoEM methods for structural biology are relatively new to many labs in India and EMBC member states. There is an intense interest in the Indian and EMBC scientific community, particularly among structural biologists, about applications of this technique, which makes this course timely. This EMBO Practical Course will deal with Single particle Cryo Electron Microscopy of biological macromolecular assemblies and Cryo Electron Tomography of Cellular structures. In addition, allied topics like MicroED is also introduced.

After the immense success of the first and second CEM3DIP CryoEM course at IISER Thiruvananthapuram in 2016 and at IIT Delhi 2018, this course will be taught for the third time in India at IISER Kolkata, and it will be the Second CryoEM EMBO Practical course. This course will be of interest to molecular biologists, cell biologists, microbiologists, structural biologists (crystallographers), theoretical/computational biologists and in general many other area biologists, and mainly aimed at generally advanced PhD students or postdoctoral researchers and faculties.

Principal themes and objectives of this EMBO PRACTICAL COURSE: CEM3DIP 2020: Single particle CryoEM of macromolecular-assemblies and cellular tomography

1) To promote the field of macromolecular structure determination by CryoEM and Cellular Tomography in India and other EMBC member states.

2) To teach scientists (biologists, structural biologists etc.) wishing to determine macromolecular or cellular structure by cryo Electron Microscopy, the basic principles and practical aspects of TEM in structural biology/structural cell biology.

3) To provide hands on practice on specimen preparation, Fourier transform in image analysis, image processing for three-dimensional reconstruction. 3D Image Processing of Macromolecular Assemblies, and cellular Tomography.

4) The main objective of this EMBO Practical Course is to enable participants (at student level, postdoc and laboratory PI level) to implement novel techniques, learnt in this course, in their home laboratories and create an avalanche effect.

Key words for this course would be ‘macromolecular-assemblies, Structural Biology / Macromolecular structure by single particle CryoEM, Cellular Tomography, MicroED, Multidisciplinary methods, Cryo EM as Hybrid methods’.

 

Speakers

 

Programme

 

Registration

  • Registration Deadline
  • 15 September 2019
  • Abstract Submission Deadline
  • 30 September 2019
  • Chosen Participants Will Be Notified By
  • 15 October 2019
  • Payment Deadline
  • 30 October 2019

 

  • STUDENT/POSTDOCS EUR 500
  • ACADEMIC EUR 500
  • INDUSTRY EUR 1250

Registration includes:

  • Accommodation
  • Meals
  • Course Material
  • Local Travel
  • Group Excursion

The registration fee does not include extra nights of accommodation before or after the course.

Payment

Payment by participants should be made to account by online bank transfer.

Accepted participants will receive a payment link. They should pay the registration fee before deadline.

Selection criteria

Selected PhD students, postdoctoral fellows and early career PIs will be expected to have the following characteristics:

  1. Project that requires application of single particle cryoEM or cryo tomography – all participants will be requested to submit abstracts for posters and submitted abstracts will be screened by a committee
  2. Having some preliminary data or exposure to electron microscopy/cryoEM/tomography will be preferred – submitted abstracts and applicant’s CV will be screened by a committee

Abstract guidelines

The abstract should be of a maximum length of 200 words. It should contain a description of the research problem that requires application of single particle CryoEM/cryo tomography. Description of preliminary data, if available, accompanied by 1 figure (maximum).

Abstract for poster
(Abstract need not be on Cryo EM, but it should have some relevance and proof that proves your need to attend this Cryo EM course. Only selected participants abstract will be printed in the abstract book) (Not exceeding 300 Words)

Poster specifications

Travel grants

A limited number of travel grants are available for participants. Applicants do not need to apply separately for travel grants for this event but should indicate on the registration form if they wish to be considered for a travel grant. Selection of awardees is handled directly by the organizer who will notify all eligible participants. More information is available at EMBO Travel Grants' page.

Child care grant

EMBO Courses and Workshops offers grants to offset additional child care costs incurred by participants or speakers when participating at any EMBO Courses and Workshop funded meeting. Eligible costs include fees for a caregiver or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please indicate on the registration form whether you would like to be considered for the grant. Please also describe how you intend to use the child care grant and specify the sum that you will need.

Additional Information

*Students will share double rooms.

*Student/Postdoctoral applicants should have to support their application with their supervisor's reference. The applicant must request his/her supervisor to send directly an email reference letter about the applicant to the email ID cem3dip2020@iisertvm.ac.in quoting the applicants Submission ID).

*CV details as requested must be filled in the form.

All selected participants will be asked to present posters on their research work, relevant to this course

* A selected set of participants may be requested to present oral presentation in addition to poster presentation

* Only selected participants will be contacted by email

 

Contact

Dr. R. Natesh or Dr. Partha P. Datta

Email: cem3dip2020@iisertvm.ac.in

Phone 0091-471-2778087 or 0091-33 663-40000 Ext:1206

 

 

Venue

Indian Institute of Science Education and Research Kolkata (IISER Kolkata) and CSIR-IICB Kolkata (on specimen preparation and data collection hands-on sessions days)

Accommodation

Accommodation will be provided at the IISER Kolkata Guest Houses. Some participants will also be accommodated at a nearby hotel (5 minutes by car). In such cases, participants will be transported to and from IISER Kolkata.

Transport

Kolkata (also known as Calcutta), the Capital city of West Bengal, is very well connected to all major international cities by air. All international flights operate out of Netaji Subhash Chandra Bose International Airport, which is 11 kilometers to the north of the city. IISER Kolkata is located approximately 40 KM from the airport and can be reached via taxis, train and public buses. People travelling from within India can also utilize the country’s vast railway network. The Howrah railway station is approximately 60 KM away from IISER Kolkata. To plan your journey go to: IISER Kolkata website

About the Area

Kolkata is a vibrant and one of the oldest cities with many historic and modern tourist attractions such as The Victoria Memorial, Fort William, Howrah Bridge, Marble Palace, Eden Gardens (cricket stadium) and a variety of museums. IISER-Kolkata is located ~ 30 miles to the north of Kolkata. An excursion to historic and modern places of Kolkata and dinner-cum river cruise on the Ganges is planned for all the course participants.

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