About the Practical Course
Production of recombinant proteins using bacterial and eukaryotic hosts is the basis of research into biological structure and function. Advances in technology and throughput in the areas of protein production and structural biology have enabled many more challenging proteins to be studied to help in understanding their function. For instance, the recent advancement of cryo-electron microscopy has made the technique suitable for a broader range of proteins whose structure could previously only be determined by X-ray crystallography. This has been particularly transformative for the challenging field of membrane protein structural biology. At this EMBO Practical Course, participants will learn our well-established high-throughput approaches for expression screening and purifying proteins for crystallization. Additionally, they will gain hands-on experience in technologies to reconstitute integral membrane proteins into detergent-free systems, such as nanodiscs, Salipro, amphipols and SMALPs, because these have been key to achieve high resolution structures by cryo-EM.