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Characterization of macromolecular complexes by integrative structural biology

12 – 19 May 2018 | Grenoble, France

  • Registration Deadline
  • 28 February 2018
  • Chosen Participants Will Be Notified By
  • 31 March 2018
  • Payment Deadline
  • 30 April 2018

Apply now!

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About the Practical Course

Practical Course illustration

This EMBO Practical Course aims to teach participants how to integrate structural biology approaches to expedite characterization of large macromolecular complexes from the atomic to cellular scale. Lectures and practical sessions will explain the techniques used to produce, purify, reconstitute and characterize multi-subunit protein and protein/nucleic acid complexes for structural analysis.

Specifically, the course will cover multi-subunit protein expression in bacteria, insect and mammalian cells; production, folding, and purification of non-coding RNAs; affinity, electrophoretic and centrifugation methods to isolate and purify sub-complexes and holocomplexes; biochemical and biophysical experiments that inform on the stoichiometric and conformational homogeneity of complexes; and strategies for determining the overall 3D architecture of large macromolecular assemblies and ultimately their structure at atomic or pseudo-atomic resolution.

Special emphasis will be placed on:

  1. the latest advances in producing targets directly from endogenous or recombinant sources or from cell free approaches (including production of RNA by in vitro transcription), and in reconstituting complexes from individually purified components or sub-complexes;
  2. techniques for assessing and improving sample quality (homogeneity, stoichiometry, monodispersity); and
  3. integration of structure determination methods (crystallography, cryo-electron microscopy, small-angle scattering, NMR , native mass spectrometry, FRET, super-resolution light microscopy) to obtain a multi-resolution view of a given complex.
 

Speakers

 

Programme

 

Registration

  • Registration Deadline
  • 28 February 2018
  • Chosen Participants Will Be Notified By
  • 31 March 2018
  • Payment Deadline
  • 30 April 2018

 

  • STUDENT/POSTDOCS EUR 250
  • ACADEMIC EUR 250
  • INDUSTRY EUR 1000

Registration includes:

  • Accommodation for 8 nights (May 12th to 20th)
  • Food
  • Course material

Students will be lodged in shared double rooms

Payment

Payment instructions will be sent to successful applicants once the selection procedure is complete.

Selection criteria

1. Priority to advanced PhD students and early-stage postdocs

2. Priority to applicants working on a challenging structural biology project involving a multi-subunit macromolecular complex


3. Preference for applicants (of any nationality) working in labs in the EMBC Member States; a limited number of applicants based in non-EMBC Member States will also be accepted

Poster specifications

Posters should preferably be printed in size A0

Travel grants

A limited number of travel grants are available for eligible participants. Applicants do not need to apply separately for travel grants for this event but should indicate on the registration form if they wish to be considered for a travel grant. Selection of awardees is handled directly by the organizer who will notify all eligible participants. More information is available at EMBO Travel Grants' page.

 

Contact

Rokhaya Tounkara

Email: gtounkar@embl.fr

Phone: 0033-(0)47620-7123

Marco Marcia

Email: mmarcia@embl.fr

 

 
venue map

Venue

EPN Science Campus

71 Avenue des Martyrs

38000 Grenoble, France

Accommodation

All participants and speakers will be lodged at the EPN Guesthouse, within walking distance of the course venue. Students will be lodged in shared double rooms

Transport

Details on reaching the EPN campus by train, plane or car can be found here.

About the Area

Touristic information regarding the city of Grenoble and its surroundings can be found here.

Sponsors

EMBO Reports 2017-08/embl_logo.jpg 2017-08/esrf_logo3.jpg 2017-08/logoibs_simple_hires.jpg 2017-08/ill_logo.jpg 2017-08/psb_logo_with_words.png 2017-11/frisbi.jpg