About the Practical Course
Two-photon imaging is the prime microscopy technique to study structural dynamics and functional signaling in cells and cellular networks of living brains. This course will teach participants the fundamental principles of multi-photon microscopy, illustrate various research applications, and provide hands-on practical experience to successfully employ this technology. Students will learn about fluorescence microscopy, microscope optics, as well as laser scanning and photodetection technology. A particular focus will be on state-of-the-art imaging approaches to study neuronal network dynamics, glia function, and neuron-glia interactions in the central nervous system of the mouse. Lab practicals will demonstrate the use of a variety of molecular probes to study cell function in vivo, including in particular fluorescent calcium indicators. The main objective of the course training is to enable participants to start their own two-photon microscopy lab.